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Comparative analysis on microbial and rat metabolism of ginsenoside Rb(1) by high-performance liquid chromatography coupled with tandem mass spectrometry.

Chen G, Yang M, Song Y, Lu Z, Zhang J, Huang H, Guan S, Wu L, Guo DA

Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 199 Guo Shoujing Road, Zhangjiang, Shanghai 201203, People's Republic of China.

Ginsenoside Rb(1) is an active protopanaxadiol saponin from Panax species. In order to compare the similarities and differences of microbial and mammalian metabolisms of ginsenoside Rb(1), the microbial transformation by Acremonium strictum and metabolism in rats were comparatively studied. Microbial transformation of ginsenoside Rb(1) by Acremonium strictum AS 3.2058 resulted in the formation of eight metabolites. Ten metabolites (M1-M10) were detected from the in vivo study in rats and eight of them were identified as the same compounds as those obtained from microbial metabolism by liquid chromatography-tandem mass spectrometry analysis and comparison with reference standards obtained from microbial metabolism. Their structures were identified as ginsenoside Rd, gypenoside XVII, 20(S)-ginsenoside Rg(3), 20(R)-ginsenoside Rg(3), ginsenoside F(2), compound K, 12beta-hydroxydammar-3-one-20(S)-O-beta-d-glucopyranoside, and 25-hydroxyl-(E)-20(22)-ene-ginsenoside Rg(3), respectively. The structures of the additional two metabolites were tentatively characterized as 20(22),24-diene-ginsenoside Rg(3) and 25-hydroxyginsenoside Rd by HPLC-MS/MS analysis. M7-M10 are the first four reported metabolites in vivo. The time course of rat metabolism of ginsenoside Rb(1) was also investigated. Copyright (c) 2008 John Wiley & Sons, Ltd.

Published 3 April 2008 in Biomed Chromatogr.
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