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Inhibitory Effects of Ginsenosides from the Root of Panax ginseng on Stimulus-Induced Superoxide Generation, Tyrosyl or Serine/Threonine Phosphorylation, and Translocation of Cytosolic Compounds to Plasma Membrane in Human Neutrophils.

He W, Liu G, Chen X, Lu J, Abe H, Huang K, Manabe M, Kodama H

jm-hkodama@kochi-u.ac.jp.

The effects of five ginsenosides (G-Rh 2, -Rd, -Rb 1, -Rb 2, -Rh 1) isolated from the root of Panax gingseng on stimulus-induced superoxide generation in human neutrophils were evaluated by measuring the reduction of ferricytochrome c. The tyrosyl or serine/threonine phosphorylation of neutrophil proteins and translocation of p47 (phox), p67 (phox), and Rac to the plasma membrane were detected using specific monoclonal antibodies. G-Rh 2 significantly suppressed superoxide generation induced by N-formylmethionyl-leucylphenylalanine (fMLP), phorbol 12-myristate 13-acetate (PMA), and arachidonic acid (AA) in a concentration-dependent manner. G-Rh 1 showed a comparably lower suppression on fMLP-induced superoxide generation. G-Rd, -Rb 1, and -Rb 2 also suppressed AA-induced superoxide generation in high concentrations. G-Rd and G-Rb 1 showed no effect on fMLP- and PMA-induced superoxide generation. FMLP-, PMA-, and AA-induced tyrosyl or serine/threonine phosphorylation and translocation of p47 (phox), p67 (phox), and Rac to the plasma membrane were in parallel with the suppression of the stimulus-induced superoxide generation.

Published 19 March 2008 in J Agric Food Chem, 56(6): 1921-7.
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