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Ginsenoside Rd production from the major ginsenoside Rb(1) by beta-glucosidase from Thermus caldophilus.

Son JW, Kim HJ, Oh DK

Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, South Korea.

Under optimum conditions (pH 5, 75 degrees C, and 0.2 U purified enzyme ml(-1)), 4 mg ginsenoside Rd was produced from 5 mg reagent-grade ginsenoside Rb(1) in 5 ml after 30 min by beta-glucosidase from Thermus caldophilus GK24. Using a ginseng root extract containing 1 mg ginsenoside Rb(1) ml(-1) and 3.2 mg additional ginsenosides ml(-1), 1.23 mg ginsenoside Rd ml(-1) was produced after 18 h; the concentrations of ginsenosides Rb(1), Rb(2), and Rc used for ginsenoside Rd production were 0.77, 0.17, and 0.19 mg ml(-1), respectively.

Published 20 February 2008 in Biotechnol Lett, 30(4): 713-6.
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