Ginseng Research Today is a free monthly online journal that collates and summarizes the latest research about Ginseng, including details on american ginseng, panax, benefits, side effects. | ||||||||
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Growth inhibition and induction of differentiation by panaxydol in rat C6 glioma cells.Hai J, Lin Q, Lu Y, Yi J, Zhang H
OBJECTIVES: Panaxydol is a naturally occurring non-peptidyl small molecule isolated from the lipophilic fractions of Panax notoginseng, a well-known Chinese traditional medicine. In this study, we aimed to investigate the effects of panaxydol on growth inhibition and its mechanisms in C6 rat glioma cells. METHODS: The effects of panaxydol on cell proliferation, morphologic changes, glial fibrillary acidic protein (GFAP) expression and cell cycle regulation in rat C6 cells were evaluated using 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, hematoxylin and eosin (HE) staining, immunocytochemistry, flow cytometric analysis and Western blot respectively. RESULTS: Panaxydol markedly inhibited the proliferation of C6 cells in a dose-dependent manner with IC(50) of 39.5 +/- 2.3 muM. In addition, the cell morphologic changes and increased expression of GFAP in C6 cells in the presence of panaxydol implied a cellular differentiation. Flow cytometric analysis revealed that panaxydol-treated cells accumulated in G(0)/G(1) phase with a marked decrease in the number of C6 cells at S phase. Western blot analysis demonstrated that panaxydol resulted in an increase in the protein expression of p27 in C6 cells as early as 3 hours after treatment consistent with the differentiation response, but protein expression of p53, p21, p16 and pRb remained unchanged. CONCLUSION: These findings suggest that panaxydol inhibits the proliferation of C6 cells via G(0)/G(1) cell cycle arrest in association with induction of p27 expression and differentiation. Published 4 September 2007 in Neurol Res.
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