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Simultaneous determination of nine saponins from Panax notoginseng using HPLC and pressurized liquid extraction.

Wan JB, Lai CM, Li SP, Lee MY, Kong LY, Wang YT

Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau, China; Department of Phytochemistry, China Pharmaceutical University, Nanjing 210038, China.

A HPLC and pressurized liquid extraction (PLE) method was developed for simultaneous determination of nine saponins, including notoginsenoside R1, ginsenoside Rg1, Re, Rf, Rb1, Rc, Rb2, Rb3 and Rd in Panax notoginseng. The analysis was performed on C18 column with water-acetonitrile gradient elution and the investigated saponins were authenticated by comparing retention time and mass spectra with their reference compounds. Several methods including PLE, ultrasonication, soxhlet extraction and immersion were used for sample preparation and their extraction efficiency was compared. The results showed that PLE has the highest extraction efficiency and repeatability, which would be valuable on standardization of sample preparation for quality control of Chinese medicines. The developed HPLC and PLE is an effective approach for simultaneously quantitative determination of sapoinins in P. notoginseng, which could be used for quality control of P. notoginseng and its preparations.

Published 20 March 2006 in J Pharm Biomed Anal, 41(1): 274-9.
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