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Pedobacter ginsengisoli sp. nov., a DNase-producing bacterium isolated from soil of a ginseng field in South Korea.

Ten LN, Liu QM, Im WT, Lee M, Yang DC, Lee ST

Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.

A Gram-negative, strictly aerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated Gsoil 104(T), was isolated from a soil sample from a ginseng field in Pocheon Province (South Korea) and was characterized taxonomically by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarities, strain Gsoil 104(T) was shown to belong to the family Sphingobacteriaceae, being related to Pedobacter africanus DSM 12126(T) (97.0 %), Pedobacter caeni LMG 22862(T) (96.9 %), Pedobacter cryoconitis DSM 14825(T) (96.8 %) and Pedobacter heparinus DSM 2366(T) (96.6 %). The phylogenetic distance from any other Pedobacter species with a validly published name was greater than 3.4 % (i.e. <96.6 % 16S rRNA gene sequence similarity). DNA-DNA hybridization experiments showed that values for DNA-DNA relatedness between strain Gsoil 104(T) and its phylogenetically closest neighbours were below 37 %. The G+C content of the genomic DNA was 43.6 mol%. The predominant respiratory quinone was MK-7. The major fatty acids were C(16 : 1)omega7c, iso-C(15 : 0), C(16 : 0), iso-C(17 : 0) 3-OH and iso-C(15 : 0) 2-OH. These chemotaxonomic data support the affiliation of strain Gsoil 104(T) to the genus Pedobacter. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 104(T) represents a novel species in the genus Pedobacter, for which the name Pedobacter ginsengisoli sp. nov. is proposed. The type strain is Gsoil 104(T) (=KCTC 12576(T)=LMG 23399(T)).

Published 3 November 2006 in Int J Syst Evol Microbiol, 56: 2565-70.
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