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Effect of oxygen supply on cell growth and saponin production in bioreactor cultures of Panax ginseng.

Trung Thanh N, Niranjana Murthy H, Yu KW, Seung Jeong C, Hahn EJ, Paek KY

Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju 361-763, Republic of Korea.

The effects of oxygen supply within the range 20.8-50% (using pure oxygen and air), on cell cultures of Panax ginseng were investigated in a balloon-type bubble bioreactor (5L capacity, containing 4L Murashige and Skoog medium, supplemented with 7.0mgL(-1) indolebutyric acid, 0.5mgL(-1) kinetin and 30gL(-1) sucrose). A 40% oxygen supply was found to be optimal for the production of both cell mass and saponin yielding values of 12.8g(DW)L(-1), 4.5mg(gDW)(-1) on day 25, respectively. Low (20.8%, 30%) and high (50%) oxygen concentration supplies were unfavorable to cell growth and saponin accumulation. The results indicate that oxygen supplementation to bioreactor-based ginseng cultures was beneficial for biomass accumulation and saponin production.

Published 27 November 2006 in J Plant Physiol, 163(12): 1337-41.
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