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Hairy root cultures of Gynostemma pentaphyllum (Thunb.) Makino: a promising approach for the production of gypenosides as an alternative of ginseng saponins.

Chang CK, Chang KS, Lin YC, Liu SY, Chen CY

Institute of Microbiology and Biochemistry, National Taiwan University, Taipei, Taiwan.

Hairy root cultures of Gynostemma pentaphyllum were established by infecting leaf discs with Agrobacterium rhizogenes. The dry biomass of hairy roots grown in MS medium for 49 days was 7.3 g l(-1) with a gypenoside content of 38 mg g(-1) dry wt.

Published 13 September 2005 in Biotechnol Lett, 27(16): 1165-9.
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The Book of Ginseng: And Other Chinese Herbs for Vitality